Paleobotany

The Evolution of Plants

From flowerless forests dominated by giant conifers to grasslands covering 40% of the Earth. The history of plants throughout the Mesozoic and Cenozoic is as dramatic as that of the dinosaurs, and the two are deeply intertwined.

~250-145 Ma Triassic and Jurassic

Forests without flowers

The plant world of the dinosaurs was radically different from ours. No grasses, no flowers, no fruits.

During the Triassic and Jurassic, the forest canopy was dominated by giant conifers of the Araucariaceae and Cheirolepidiaceae families, reaching 30 to 60 meters. Below, cycads, bennettitales, and ginkgoales formed the mid-story, and the ground was covered by ferns, mosses, and horsetails. There were no grasses, flowers, or fruits. This landscape persisted for over 100 million years.

Sauropod diets depended on these plants. Carbon isotope analyses from tooth enamel (Morrison Formation, USA) indicate that Brachiosaurus reached the araucaria canopy, while Diplodocus grazed on ground-level ferns. Laboratory tests (Hummel et al., 2008) showed that Araucaria foliage and horsetails produce energy comparable to modern fodder, making the gigantism of these animals viable.

Notable fossil sites

Petrified Forest, Arizona (~225 Ma): silicified trunks of Late Triassic conifers.

Cerro Cuadrado, Patagonia (~160 Ma): Araucaria mirabilis cones with cellular preservation and intact embryos.

Skåne, Sweden: Jurassic ferns with preserved cell nuclei and chromosomes (Bomfleur et al., 2014).

Silicified cone of Araucaria mirabilis, Middle Jurassic, Cerro Cuadrado, Argentina

Silicified cone of Araucaria mirabilis, Middle Jurassic (~160 Ma), Cerro Cuadrado, Patagonia. Extraordinary cellular preservation with intact embryos. Wikimedia Commons.

Jurassic forest vs. modern

Ground cover Ferns, mosses, horsetails
Flowers None
Fruits None
Canopy Araucarias (30-60 m)
Pollination Wind (primarily)
Atmospheric CO₂ ~1,000-2,000 ppm
~237-66 Ma Triassic to Cretaceous

The "flowers" before flowers: Bennettitales

An extinct order that developed flower-like structures completely independently from angiosperms. A remarkable case of convergent evolution.

Bennettitales were an order of gymnosperm plants that lived for nearly 170 million years. Externally resembling modern cycads, with robust trunks and pinnate leaves, they concealed a crucial difference: their reproductive organs. Some species, such as Williamsonia and Cycadeoidea, developed flower-like structures with petaloid bracts and male and female organs united in the same reproductive unit.

The decline of Bennettitales is closely tied to the rise of angiosperms. As flowering plants diversified from ~100 Ma onward, they conquered the ecological niches previously occupied by Bennettitales. With faster growth rates and mutualistic relationships with pollinators, angiosperms held a decisive competitive advantage. Bennettitales disappeared at the K-Pg boundary (66 Ma), possibly already in severe decline before the Chicxulub impact.

Convergent evolution

The former "Anthophyte Hypothesis" proposed that Bennettitales were direct ancestors of flowering plants. Modern phylogenetic analyses refuted this idea: Bennettitales are closer to cycads and ginkgos, not to angiosperms. The floral resemblance is purely convergent, which makes the group even more fascinating.

Two families

Williamsoniaceae

Branching trunks, exposed "flowers" with petaloid bracts. Visually similar to angiosperm flowers.

Cycadeoidaceae

Compact barrel-shaped trunks. Reproductive structures embedded in the surface, often closed (cleistogamy).

~136-90 Ma Early to Late Cretaceous

The "abominable mystery": the angiosperm revolution

Charles Darwin, in an 1879 letter to Joseph Hooker, called the explosive emergence of flowering plants an "abominable mystery." In 30 million years, they went from marginal to dominant.

"The rapid development as far as we can judge of all the higher plants within recent geological times is an abominable mystery."

Charles Darwin, letter to Joseph Hooker, July 22, 1879

The earliest angiosperm fossil records include pollen grains from ~136 million years ago found in Israel, the aquatic plant Montsechia vidalii (~130 Ma, Spain), and Archaefructus sinensis (~125 Ma, China). In just 30 million years, they went from marginal elements to dominants in terrestrial ecosystems, one of the fastest ecological transitions in history.

The impact on fauna was profound. The diversification of ornithischian dinosaurs in the Late Cretaceous coincides with angiosperm dominance. Hadrosaurs ("duck-billed" dinosaurs) developed complex dental batteries, with hundreds of compacted teeth forming grinding surfaces to process the tough, silica-rich leaves of the new plants.

Earliest fossils

~136 Ma

Angiosperm pollen grains in Israel and Switzerland

~130 Ma

Montsechia vidalii, aquatic plant from Spain (Gomez et al., 2015)

~125 Ma

Archaefructus sinensis, Yixian Formation, China (Sun et al., 2002)

Decisive adaptations

Xylem vessels: more efficient water transport than conifer tracheids

Broad leaves: higher vein density, maximizing photosynthesis

Insect pollination: precise reproduction and accelerated speciation

Fruits: seed dispersal by animals, colonizing new habitats

Double fertilization: produces endosperm only when fertilization occurs, conserving energy

~120-80 Ma Mid to Late Cretaceous

Flowers and pollinators: coevolution

The relationship between flowers and pollinating insects is one of the most successful partnerships in evolution. And it began in the world of dinosaurs.

The earliest evidence of insect pollination dates back to ~99 million years ago, preserved in Burmese amber (Myanmar). Fossils of thrips (Gymnopollisthrips minor) carrying pollen grains and beetles (Cretoparacucujus cycadophilus) associated with cycad pollen demonstrate that entomophilous pollination arose even before the great angiosperm diversification. Long-proboscid flies also appear in this period.

Insect pollination dramatically accelerated angiosperm diversification. By enabling reproductive isolation and rapid speciation, each flower-pollinator pair could diverge independently. Molecular clock studies (Magallón et al., 2015) show that the major angiosperm lineages (rosids, asterids) diversified between 115 and 90 Ma, coinciding with pollinator diversification.

Progression of pollination syndromes

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Beetles

Oldest; visiting gymnosperm cones

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Flies

Long proboscises in the mid-Cretaceous (~99 Ma)

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Bees

Melittosphex burmensis (~100 Ma), the oldest known bee

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Butterflies and moths

Moths with proboscis since ~130 Ma; butterflies in the Paleogene

Key evidence: Burmese amber (~99 Ma)

Multiple insect groups preserved carrying pollen: thrips, beetles, flies. Myanmar amber is Cenomanian in age and also contains one of the oldest flowers preserved in resin, Micropetasos burmensis, with structures indicating insect pollination.

66 Ma Cretaceous-Paleogene boundary

The fern spike: signature of catastrophe

When everything died, ferns were the first to return. The "fern spike" is the clearest palynological signal of ecological collapse in the fossil record.

First identified by Tschudy et al. (1984) in rocks from the Hell Creek Formation (Montana, USA), the "fern spike" consists of an abrupt increase in fern spores in the palynological record: from about 25% to over 70 to 100% of palynomorphs, just above the iridium layer deposited by the Chicxulub impact. Vajda and Bercovici (2014) confirmed the pattern on a global scale, documenting equivalent fern spikes in New Zealand and Japan.

The K-Pg fern spike lasted between 1,000 and 10,000 years, an extremely brief geological interval that reveals the scale of the devastation. Analogous phenomena were recorded after the end-Permian extinction (~252 Ma), with lycophytes dominating the record, and at the end of the Triassic (~201 Ma). Full recovery of diversified forests took between 100,000 and 1,000,000 years.

Why do ferns dominate after catastrophes?

Microscopic spores: produced by the millions, wind-dispersed, no dependence on pollinators

Tolerate low light: survive the "impact winter" caused by atmospheric dust

Rapid colonization: grow in poor, acidic, and newly exposed soils

No animal dependence: reproduction completely independent of fauna

Post-impact recovery sequence

Year 0

Vegetation collapse, iridium layer

~1-10k years

Fern spike: ferns dominate the landscape

~100k years

Gradual return of angiosperms and conifers

~1 million years

Full recovery of diversified forests

Modern analogues: after the eruption of Krakatoa (1883) and Mount St. Helens (1980), ferns were among the first plants to establish themselves on volcanic ash.

And in this world of conifers, flowers, and ferns, one family of plants was quietly beginning its rise...

The rise of grass

From a marginal plant in the Cretaceous to the dominant biome in the Miocene: the trajectory of grasses over 100 million years.

~100-80 Ma Mid-Late Cretaceous

Origin of grasses

Molecular estimates place the origin of the Poaceae family between 80 and 100 million years ago, still in the Cretaceous period. The oldest grass fossil was found in Myanmar amber (~100 Ma). The first grasses were small understory plants, growing in the shade of conifers, ferns, and angiosperms that dominated the Mesozoic landscape.

~67 Ma Late Cretaceous (Maastrichtian)

Dinosaurs ate grass

In 2005, Prasad and colleagues published a discovery in Science that shook paleontology: phytoliths from at least five different types of grasses were found in titanosaur coprolites from the Lameta Formation, central India. Giant sauropods of the Late Cretaceous were already including grasses in their diet. Grasses were not dominant, but they were part of the available vegetation.

66 Ma Cretaceous-Paleogene boundary

Grasses survive the extinction

The Chicxulub impact killed the non-avian dinosaurs, but grasses made it through the catastrophe. As herbaceous plants with basal meristems (growth points close to the ground), they were more resistant to destruction than trees. This same trait makes grasses resistant to fire, grazing, and drought.

66-34 Ma Paleocene and Eocene

Silent diversification

In the warm, humid world of the Paleocene and Eocene, forests dominated. Grasses remained minor components of the vegetation for tens of millions of years, slowly diversifying in understories and along riverbanks. The Bambusoideae and Ehrhartoideae subfamilies (which include rice) differentiated during this period. The phytolith record shows a gradual increase in grass presence.

34-23 Ma Oligocene

First open grasslands

Global cooling after the Antarctic glaciation (~33.9 Ma) and falling CO₂ levels created drier, more seasonal conditions. Forests retreated. For the first time, open habitats dominated by grasses began to appear in parts of South America and Africa. The phytolith record shows the transition from forest vegetation to mixed.

23-5 Ma Miocene

The Grass Revolution

The great moment. Global cooling, the drop in atmospheric CO₂ below ~500-800 ppm, and increased seasonality created ideal conditions for the explosion of C4 grasses. Open grasslands replaced forests across vast continental areas of Africa, the Americas, and Asia. Fauna was transformed: horses evolved from three-toed forest animals to single-hoofed grazers with hypsodont teeth (high-crowned, resistant to silica wear from grasses). Ruminants diversified. Swift predators thrived on the open plains. It was the greatest restructuring of terrestrial ecosystems since the extinction of the dinosaurs.

Today

The dominant biome

Grasses cover approximately 40% of the Earth's land surface (excluding Greenland and Antarctica). The Poaceae family has about 12,000 species. Wheat, rice, corn, sorghum, sugarcane, and rye are all grasses: they sustain the caloric foundation of human civilization. Natural grasslands are now considered the most threatened biome on the planet, with over 70% already converted to agriculture.

Did dinosaurs eat grass?

For decades, paleontology assumed that grasses only appeared after the dinosaurs. In 2005, titanosaur coprolites in India proved otherwise.

What are phytoliths?

Phytoliths are microscopic silica (SiO₂) structures that form inside plant cells. Each grass subfamily produces phytoliths with distinct diagnostic shapes: bilobate, saddle-shaped, cross-shaped. When the plant dies or is digested, phytoliths resist decomposition and remain in the soil, sediments, or coprolites for millions of years. They are like botanical "fingerprints" that survive geological time.

What they found

In the coprolites from the Lameta Formation (central India, ~67 Ma), Prasad et al. identified phytoliths from at least five distinct grass lineages, including representatives of the Bambusoideae and Ehrhartoideae subfamilies (the rice subfamily). The diversity found indicates that the family was already well differentiated before the extinction of the dinosaurs. In 2011, a second study by the same group, published in Nature Communications, demonstrated that the rice tribe (Oryzeae) had already diversified in the Late Cretaceous.

Before vs. after the discovery

Old view

"Grasses arose in the Cenozoic, after the extinction of the dinosaurs. No dinosaur ever ate grass. Open grasslands are a post-dinosaur phenomenon."

Current view

"Grasses arose in the Cretaceous (80-100 Ma) and coexisted with dinosaurs for at least 30 million years. Titanosaurs ate grass. Open grasslands came later, in the Miocene."

Grass phytoliths observed under scanning electron microscope (SEM)

Grass phytoliths observed under scanning electron microscope (SEM). These silica structures are preserved for millions of years and allow identification of grass subfamilies in fossil coprolites. Wikimedia Commons (CC BY-SA 3.0)

Elongated grass phytoliths observed under optical microscope

Elongated grass phytoliths under optical microscope. The diagnostic shapes (bilobate, saddle, cross, elongated) vary by subfamily, functioning as botanical "fingerprints." Wikimedia Commons (CC BY-SA 3.0)

C4 Photosynthesis: the secret weapon

Most plants use the C3 photosynthetic pathway. Some grasses developed something better.

What is the C4 pathway?

A modified photosynthetic mechanism that concentrates CO₂ around the RuBisCO enzyme, reducing photorespiration. It is more efficient than the C3 pathway under conditions of intense heat, high light, drought, and low atmospheric CO₂.

Extreme convergent evolution

C4 photosynthesis evolved independently at least 22 to 24 times within grasses, one of the most remarkable examples of convergent evolution in plants. The selective pressure from falling CO₂ in the Oligocene-Miocene favored this mechanism in multiple lineages simultaneously.

Disproportionate impact

C4 grasses represent only ~3% of plant species but account for 23-25% of global terrestrial primary productivity. They dominate tropical and subtropical savannas. Corn, sugarcane, sorghum, and millet are all C4.

How grasses changed the Earth

A plant family that reshaped ecosystems, soils, fire regimes, and the evolution of animals.

Grass-fire cycle

Grasses produce dry, continuous, and flammable biomass that burns easily and regenerates rapidly from underground meristems. Fire kills young trees, but grasses resprout in weeks. This creates a feedback loop: more grasses generate more fire, which prevents forest encroachment, which favors more grasses. The expansion of grasslands in the Miocene coincides with increased charcoal deposits in the sedimentary record.

The most fertile soils on the planet

Grasses possess dense, fibrous root systems that penetrate up to 2-3 meters deep. These roots contribute massive amounts of organic carbon to the soil, creating the world's most fertile soils: Mollisols (Chernozems). The North American Great Plains, the Ukrainian steppe, and the Argentine Pampas owe their fertility to millions of years of organic matter accumulation by grasses.

Faunal transformation

The Miocene Grass Revolution restructured fauna on every continent. Horses evolved from Mesohippus (3 toes, low teeth for leaves) to Equus (single hoof, hypsodont teeth for abrasive silica-rich grasses). Ruminants such as bovids and cervids diversified with multi-chambered stomachs to digest cellulose. Cursorial predators (runners) like felids and canids thrived on the open plains.

Grasses by the numbers

~40%

of the land surface

12,000

known species

100 Ma

oldest fossil

22-24x

times C4 evolved independently

5+

grass types in titanosaur coprolites

>70%

of native grasslands already lost

References

Grasses

Prasad, V., Strömberg, C.A.E., Alimohammadian, H. & Sahni, A. (2005). Dinosaur coprolites and the early evolution of grasses and grazers. Science, 310(5751), 1177-1180.

Strömberg, C.A.E. (2011). Evolution of grasses and grassland ecosystems. Annual Review of Earth and Planetary Sciences, 39, 517-544.

Cerling, T.E. et al. (1997). Global vegetation change through the Miocene/Pliocene boundary. Nature, 389(6647), 153-158.

Prasad, V. et al. (2011). Late Cretaceous origin of the rice tribe provides evidence for early diversification in Poaceae. Nature Communications, 2: 480.

Mesozoic forests and dinosaur diet

Hummel, J. et al. (2008). In vitro digestibility of fern and gymnosperm foliage: implications for sauropod feeding ecology. Proc. R. Soc. B, 275(1638), 1015-1021.

Bomfleur, B., McLoughlin, S. & Vajda, V. (2014). Fossilized nuclei and chromosomes reveal 180 million years of genomic stasis in Royal Ferns. Science, 343(6177), 1376-1377.

Bennettitales

Crane, P.R. (1985). Phylogenetic analysis of seed plants and the origin of angiosperms. Annals of the Missouri Botanical Garden, 72(4): 716-793.

Crepet, W.L. (1974). Investigations of North American cycadeoids: the reproductive biology of Cycadeoidea. Palaeontographica Abt. B, 148: 144-169.

Angiosperm revolution

Gomez, B. et al. (2015). Montsechia, an ancient aquatic angiosperm. PNAS, 112(35), 10985-10988.

Sun, G. et al. (2002). Archaefructaceae, a new basal angiosperm family. Science, 296(5569), 899-904.

Flower-pollinator coevolution

Poinar, G.O. & Danforth, B.N. (2006). A fossil bee from Early Cretaceous Burmese amber. Science, 314(5799), 614.

Peñalver, E. et al. (2012). Thrips pollination of Mesozoic gymnosperms. PNAS, 109(22), 8623-8628.

Fern spike

Tschudy, R.H. et al. (1984). Disruption of the terrestrial plant ecosystem at the Cretaceous-Tertiary boundary. Science, 225(4666), 1030-1032.

Vajda, V. & Bercovici, A. (2014). The global vegetation pattern across the Cretaceous-Paleogene mass extinction interval. Global and Planetary Change, 122, 29-49.